After so much preparation and hard work, this is the key event which every person going through IVF treatment hopes will go well. It is usual practice for the male partner to have produced a sample of sperm beforehand which has been frozen in readiness. Alternatively, a fresh sample is collected just before the insemination process. Understandably, some men feel under too much pressure to perform and produce the semen required. This is why using semen which has been frozen is a practical alternative. It also allows for couples to be together when the eggs are collected, if this is an option offered at the particular fertility clinic.
The semen needs to be prepared and washed to remove any impurities which may interfere with fertilisation. The sperm are then temporarily stored in a culture medium until they are mixed with the eggs which have already been collected. They are then carefully placed into a special incubator to work their magic with each other.
Around 18-24 hours after the eggs and sperm have been combined they are examined to see if fertilisation has occurred. A specialist scientist, known as an embryologist, will look to see if there are two clear areas inside the egg. One will be the nucleus of the egg from the mother and the other will be the nucleus from the sperm of the father. This is the point where the genes from each parent combine and is known as “pro nuclei development”.
The general time frame is around 2 days after fertilisation occurring, for the embryo or embryos to be transferred into the woman’s uterus or into one of her fallopian tubes. Then it is a matter of waiting and seeing until a pregnancy test indicates if the embryo has implanted. This can be a very long two week wait with couples anxiously biding their time hoping on a positive result.
This is a highly specialised procedure and is done in cases of male infertility, when the numbers and quality of sperm are not ideal. A single sperm is injected directly into an egg with a glass pipette which is so fine, the end is too small to see. With the aid of a microscope, the scientist inserts the end of the pipette straight into the cytoplasm of the egg.
Because it is such delicate work, the table, microscope and the equipment all need to be specially stabilised to prevent any movement from hindering the process. It is also important that the nucleus of the sperm and egg are not damaged and only a single, ideal sperm is injected. If it has been successful, the cells will start to divide within the next 24 hours.
Freeze storage of embryos is done when there is an excess and they may be needed for future cycles. Transferring thawed embryos which have been frozen is, again, a complex process and not all embryos will survive the thawing process. Only those which are assessed to be in good condition and which will offer the best chance of a pregnancy are transferred. Fertility clinics generally charge a fee to store embryos.
The success of thawing and transferring the embryo depends on a number of factors. The age of the woman when the eggs were originally collected is important as is the reason why there were infertility issues in the first place. So is the quality of the eggs and their stage of development when they were originally frozen. The number of frozen embryos will also play a part in the success of how well they stand up to being thawed and then be successfully transferred.
Not every couple will use all of their frozen embryos. This means they can need to make a decision about what to do with them; either giving permission to the fertility clinic for them to be disposed of or used for research purposes. Alternatively, the embryos can be donated to other infertile couples.
Each fertility clinic has its own practices but generally the arrangement of couple matching exists. This is where both couples approve each other as being a donor or recipient of the embryos. This process is done confidentially or only if both consent, to contact with each other. Medical histories, hair and eye colour and genetic characteristics all help when couples make their decisions about who may be the donor or recipient of the embryos.
Most fertility clinics operate a waiting list for couples who want to become embryo recipients. This is a realistic option for singles who wish to have a child or same sex couples. It also offers a solution for couples who have experienced such significant fertility issues that using their own eggs and sperm are not an option.
At every stage of the IVF process, clinics have to be extremely careful about matching sperm and eggs with the correct individuals. Systems of checking, cross checking and checking again are routine and couples are often asked to identify their names on collection tubes and laboratory equipment. These processes are necessary to ensure that there are no mistakes made which could inadvertently result in a pregnancy which is not genetically matched.
Pregnancy rates vary from clinic to clinic. They usually include success rates on their internet sites and in the written material they give to couples when doing preliminary research into fertility.
Within Australia there is inconsistency between the states and territories regarding legislation of reproductive technology. Each designs and implements separate legislation. Reproductive Technology Acts and Regulations are listed on The National Health and Medical Research Council www.nhmrc.gov.au web-site.